Evaluation of antidiabetic activity of ipomoea aquatica. We therefore examined the hepatic iron content, serum iron parameters, intestinal iron absorption, and liver hepcidin expression in rats treated with. Diabetic rats 200 mgdl were divided in 7 groups 3 rats per group and maintained in separate cages for 5 h, 10 h, 24 h, 36 h, 48 h, 5 days and 10 days after stz injection. Anti diabetic effect of sorghum extract on hepatic gluconeogenesis of streptozotocininduced diabetic rats by jungmin kim and yongsoon park download pdf 693 kb. Wound healing in streptozotocininduced diabetic rats. This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocininduced diabetes that were chronically exposed to f. Does honey have any salutary effect against streptozotocin. The onset speed of hyperglycemia is important to the development of neuropathic hyperalgesia in streptozotocin. Diabetic rats had significantly impaired behavioural and cognitive functions. Therefore, the purpose of this study was to assess the hypoglycemic effect of the aqueous extract of c. Does honey have any salutary effect against streptozotocin induced diabetes in rats. Preclinical evaluation of antihyperglycemic activity of. Challenges and issues with streptozotocininduced diabetes a clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics author links open overlay panel sameer n.
Streptozotocin or streptozocin inn, usp stz is a naturally occurring alkylating antineoplastic agent that is particularly toxic to the insulinproducing beta cells of the pancreas in mammals. Diabetes was induced in young, weaned, agematched rat pups by streptozotocin. The present study was aimed to examine the hypoglycemic and insulinsensitizing capacity of onion peel extract ope containing high quercetin in high fat dietstreptozotocininduced diabetic rats and to elucidate the mechanism of its insulin. Antihyperglycemic and antihyperlipidemic activities of. Gynura procumbens, an herb with known anti diabetic properties, was used in this investigation to determine the effect of its aqueous and ethanolic extracts on sperm quality and testosterone level of male streptozotocininduced type 1 diabetic rats. Diabetes mellitus was induced by single intraperitoneal injection of 35.
Several studies have reported that some parts of the c. As many as 15% of people with diabetes will develop foot ulceration and wounds, and 3% will require lower limb amputation. Male wistar rats 60 days old were randomly distributed into two groups of 18 animals. In diabetic rats, the number and size of islets was reduced and the immunoreactivity for insulin was diminished. Iron overload is frequently observed in type 2 diabetes mellitus dm2, but the underlying mechanisms remain unclear. Streptozotocin stz is an antibiotic that produces pancreatic islet. Diabetes mellitus is one of the major health problems all over the world. However, the mechanisms mediating the biological activity of these products are still unclear. Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats. Results of in vitro and in vivo analyses in rats treated with leaf extract or agnps show that both the extract and the biologically synthesized agnps have antidiabetic activity. The effect of rosuvastatin and simvastatin therapy 10 mgkg for 5 weeks on bloodbrain barrier bbb, bloodretinal barrier brb, and cardiac muscle permeability of streptozotocininduced diabetic rats was studied.
The mechanism of alloxan and streptozotocin action in b. Streptozotocininduced diabetes in the rat is associated. Diabetes was induced in rats with equal molar dosages of either streptozotocin or alloxan. Quercetin derivatives in onions have been regarded as the most important flavonoids to improve diabetic status in cells and animal models. Administration of high doses of fluoride f can alter glucose homeostasis and lead to insulin resistance ir. Streptozotocininduced diabetic models in mice and rats.
Histology and immunohistochemistry of the pancreatic islets of langerhans in control left panel and streptozotocininduced diabetic right panel rats. Hypoglycemic effects of gynura procumbens fractions on. In this study, we used an argonbased round atmosphericpressure plasma jet appj for enhancing wound healing in streptozotocin stz induced diabetic rats. Traditional plant treatment for diabetes has shown a surging interest in the last few decades.
Objective the present study was designed to evaluate the diabetes induced neuronal damage in the hippocampus of the rat brain. The stzinjected rats exhibited hyperglycaemia accompanied with weight loss, indicating their diabetic condition. Therefore, four groups of rats were allocated as control c, stzinduced dm d, and. Effects of duration of diabetes on behavioural and cognitive. The most frequently used intravenous dose of this drug to induce diabetes in rats is 65 mgkg b. Regulation of podocalyxin expression in the kidney of. Described in this unit are two protocols used to produce stzinduced diabetes in mice basicprotocol1andratsbasicprotocol2. Alhagi maurorum camel thorn plant is a promising medicinal plant due to the presence of flavonoids and phenolic compounds as major contents of its constituents. Diabetes is associated with declining sexual function in women.
The sizeselective permeability of different vascular beds to a group of fluorescein isothiocyanate dextrans. The clinical course of the diabetes mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. Antidiabetic properties and mechanism of action of orthosiphon stamineus enth bioactive subfraction in streptozotocininduced diabetic rats. Metformin and glibenclamide were used as control drugs in this study. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin stzinduced diabetic rats and suggested.
Statins ameliorate endothelial barrier permeability. In this study, we examined the effects of hippophae rhamnoides l. Detailed in this unit are protocols for producing stz. The streptozotocin diabetic rat as a model of the chronic complications of human diabetes michael wei1 bsc, leslie ong1 bsc, maree t. After 4 weeks of feeding on a highfat diet, rats were injected intraperitoneally with streptozotocin to. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized.
Streptozotocin enters the b cell via glucose transporter glut 2 and causes. Streptozotocininduced diabetic ratsmice stz model advantages. The onset speed of hyperglycemia is important to the. The neurological consequences of diabetes mellitus in the brain have gained attention most recently. Bauhinia holophylla leaves treatment on the streptozotocininduced diabetic rats. Proteomic analysis of gastrocnemius muscle in rats with. This study tested the hypothesis that dietary arginine supplementation increases endothelial tetrahydrobiopterin bh 4 availability for nitric oxide no synthesis in diabetic rats. Biochemical effects of oleuropein in streptozotocin. Diabetes mellitus was induced in male spraguedawley rats weighing 200220 g by an injection of streptozotocin stz dissolved in a citrate buffer into the tail vein at a dose of 45 mgkg. Challenges and issues with streptozotocininduced diabetes. Antidiabetic activity of different extracts of myrtus. Antidiabetic activity of different extracts of myrtus communis in. Pdf brain indoleamines in alloxan and streptozotocin. The aim of this study was to investigate potential hypoglycemic and hypolipidemic effects of protodioscin in diabetic rats.
Histopathological and immunohistochemical study of antidiabetic. Sbt on the plasma blood glucose and cholesterol level in diabetic rats. It is used in medicine for treating certain cancers of the islets of langerhans and used in medical research to produce an animal model for hyperglycemia and alzheimers in a large dose, as well as. Induction of diabetes by streptozotocin in rats springerlink. Diabetes is a complex metabolic disorder involving many organs and can devastate the lives of affected individuals. Serum and liver lipids distributions in streptozotocin induced.
Streptozotocininduced experimental diabetes in male. Preclinical evaluation of antihyperglycemic activity of clerodendron infortunatum leaf against streptozotocininduced diabetic rats, diabetes therapy, 2011, pp. Rapamycin protects against hepatic injury in streptozotocininduced. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. Pdf effect of streptozotocin induced diabetes on rat. Streptozotocininduced diabetic rat model background strain. Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. Streptozotocin stz is toxic to pancreatic islet insulinproducing bcells, thus inducing diabetes.
Streptozotocininduced diabetic rats either were given unrestricted access to a caseinbased diet expt. Effect of bauhinia holophylla treatment in streptozotocininduced. Limiting prolonged inflammation during proliferation and. Request pdf streptozotocininduced diabetic models in mice and rats streptozotocin stz is an antibiotic that can cause pancreatic. Comparison of streptozotocin and alloxaninduced diabetes. Wistar rat, male, 250g body weight streptozotocin stz induction protocol. There are several successful chinese herbal products for the treatment of patients with diabetic nephropathy in china. Biochemical effects of oleuropein in streptozotocin induced diabetic male rat. The aim of this study was to assess the behaviour in streptozotocininduced diabetic wistar rats and to evaluate the behavioural effects of caffeine administration. Currently, stz is most often used to induce diabetes in rats and mice. Gynura procumbens, a medicinal plant locally known as sambung nyawa, is widely used traditionally in south east asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Adult female wistar rats were divided into four groups n 6group.
In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. At a dose of 500 mgkg, raw ginger was significantly effective in lowering serum glucose, cholesterol and triacylglycerol levels in the gingertreated diabetic rats compared with the control diabetic rats. If the inline pdf is not rendering correctly, you can download the pdf file here. Dietary larginine supplementation enhances endothelial.
Diabetes was induced by streptozotocin 40 mgkg in female wistar rats. Antidiabetic and hypolipidaemic properties of ginger. Effects of stzinduced diabetes on the relative weights of. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in the diabetic renal complications.
Intravenous injection of 60mgkg dose of streptozotocin in adult wistar rats, makes pancreas swell. Antihyperglycemic effect of an extract of myrtus communis in streptozotocininduced diabetes in mice. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, cpeptide and body weight decreases. Human islets are considerably more resistant to alloxan than those of the rat and mouse eizirik et al. The stz model provides a good, simple screening tool to test compounds and to further understand the complex development of neuropathic pain. Novel insight into streptozotocininduced diabetic rats. The objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and cpeptide in serum, between normal and diabetic rats. Onion peel extracts ameliorate hyperglycemia and insulin. Other models of diabetes include genetic models and the use of a prolonged highfat diet regimen. Background diabetes mellitus is an endocrine metabolic disorder of impaired carbohydrate, fat and protein metabolisms characterized by chronic hyperglycemia. It also changes normal metabolism in diabetic rats in comparison with normal rats. Hoey4 phd, darryl burstow5 mbbs fracp and lindsay brown1 phd departments of physiology and pharmacology1 and pharmacy2, the university of queensland. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin.667 1095 1435 1379 915 326 1502 816 435 1011 463 1380 1259 1367 46 544 1065 947 974 772 289 714 599 555 466 812 840 98 1288 694 527 362 930 243 1366 985 12 228 1215 1357 1130 366 552 1456 939